Transmission of radiation-induced rearrangements through cell divisions

A BrdU incorporation technique associated with heat denaturation, acridine orange staining and UV irradiation, was applied to G0-irradiated lymphocyte cultures. This made it possible both to obtain an R-banding, and to estimate the number of divisions undergone by each cell in mitosis irradiation. C...

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Bibliographic Details
Published inMutation research Vol. 198; no. 1; p. 191
Main Authors Al-Achkar, W, Sabatier, L, Dutrillaux, B
Format Journal Article
LanguageEnglish
Published Netherlands 01.03.1988
Subjects
Cell Cycle - radiation effects
Cell Division
Chromosome Aberrations
Chromosome Banding
DNA Damage
Humans
Lymphocytes - cytology
Lymphocytes - radiation effects
Lymphocytes - ultrastructure
Sister Chromatid Exchange - radiation effects
Ultraviolet Rays
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Summary:A BrdU incorporation technique associated with heat denaturation, acridine orange staining and UV irradiation, was applied to G0-irradiated lymphocyte cultures. This made it possible both to obtain an R-banding, and to estimate the number of divisions undergone by each cell in mitosis irradiation. Cell survival and slowing down of the cell cycle could be distinguished. The frequency of various types of rearrangements, and their association was studied at each cell division. It is shown that the loss of cells carrying chromosomal rearrangements is determined by several parameters such as the presence of dicentric or multicentric chromosomes and above all the association of several rearrangements in the same cell.
ISSN:0027-5107
DOI:10.1016/0027-5107(88)90054-1