Silanization of glass in the making of ion-sensitive microelectrodes

The silanization of glass, particularly Pyrex, was studied using reaction conditions that might be applied in the fabrication of ion-sensitive microelectrodes of the liquid-membrane type. The efficacy was tested by measuring the hydrophobicity (contact angle) or electrical resistivity of the treated...

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Bibliographic Details
Published inJournal of neuroscience methods Vol. 8; no. 3; p. 231
Main Authors Munoz, J L, Deyhimi, F, Coles, J A
Format Journal Article
LanguageEnglish
Published Netherlands 01.07.1983
Subjects
Animals
Electrolytes - metabolism
Ion Channels - physiology
Membrane Potentials
Microelectrodes
Neurons - physiology
Neurophysiology - instrumentation
Silanes
Silicon
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Summary:The silanization of glass, particularly Pyrex, was studied using reaction conditions that might be applied in the fabrication of ion-sensitive microelectrodes of the liquid-membrane type. The efficacy was tested by measuring the hydrophobicity (contact angle) or electrical resistivity of the treated surface. Aminosilanes, such as trimethyl-(dimethylamino)-silane are better than chlorosilanes, the optimum temperature is 250-330 degrees C, and the reaction comes near to completion in 5 min. Silanization of glass that is newly exposed (as in the pulling of a micropipette) is greatly improved if the surface is treated with acid. There is considerable variation from one kind of glass to another. A recipe for making double-barrelled ion-sensitive microelectrodes is given.
ISSN:0165-0270
DOI:10.1016/0165-0270(83)90037-7