Hemato-immunological and biochemical parameters of silver catfish Rhamdia quelen immunized with live theronts of Ichthyophthirius multifiliis

• Published in: Fish & shellfish immunology Vol. 45; no. 2; pp. 689 - 694
• Main Authors: Tancredo, Karen R., Gonçalves, Eduardo L.T., Brum, Aline, Acchile, Monyele, Hashimoto, Gabriela S.O., Pereira, Scheila A., Martins, Maurício L.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2015
• Subjects: Animals; Antibodies, Protozoan - blood; Antibodies, Protozoan - immunology; Blood Cell Count; Catalase; Catalase - metabolism; Catfishes - blood; Catfishes - immunology; Catfishes - parasitology; Ciliophora; Ciliophora Infections - blood; Ciliophora Infections - immunology; Ciliophora Infections - parasitology; Ciliophora Infections - veterinary; Fish; Fish Diseases - blood; Fish Diseases - immunology; Fish Diseases - parasitology; Gills - parasitology; Hematology; Host-Parasite Interactions; Hymenostomatida; Ichthyophthirius; Ichthyophthirius multifiliis; Immunoglobulin; Liver - immunology; Liver - metabolism; Protozoan Vaccines; Rhamdia quelen; Theronts; Vaccination - veterinary; Vaccine; White spot disease; Theronts; Catalase; Hematology; Ciliophora; White spot disease; Fish; Vaccine; Immunoglobulin
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2015.05.024

This study investigated the immunization by intraperitoneal injection (i.p.) (assay I) and immersion bath (assay II) with live theronts of Ichthyophthirius multifillis in Rhamdia quelen and its influence on the hemato-immunological and biochemical parameters. Fish were divided in control (non immunized no challenged); non immunized and challenged with 12,000 theronts/fish; non immunized and challenged with 22,000 theronts/fish; immunized and challenged with 12,000 theronts/fish; immunized and challenged with 22,000 theronts/fish. Six days after challenge, either in the assay I or in the assay II the prevalence of I. multifillis in the gills was higher in non immunized fish (33.33% and 27.77%, respectively). In the assay I showed higher numbers of thrombocytes, leukocytes, lymphocytes, neutrophils and monocytes 20 days after injection and lower numbers after challenge. The immunoglobulin values were higher in fish non immunized. Fish immunized by immersion bath (assay II) showed greater values of catalase (CAT) in the liver (1245.49 U/mgprt) when compared to i.p. (198.79 U/mgprt). The levels of CAT in the liver of fish from the assay II were greater (1738.47 U/mgprt) 14 days after immunization than that observed 21 days after (1114.26 U/mgprt). The vaccination by i.p method showed influence on the hematological parameters. On the other hand, the immersion bath vaccination showed greater influence on the catalase activity in the liver. The results showed that new parameters like total protein, immunoglobulin and antioxidant enzymes could be considered in evaluating the host response to infection. [Display omitted] •The South American silver catfish was immunized with theronts via i.p. and immersion bath.•Vaccination via i.p. increased the number of leukocytes, thrombocytes, lymphocytes and monocytes.•Vaccination via immersion bath increased the catalase activity in the liver of fish.•Total protein and catalase are parameters to be considered in immunization assays.