Reduced cytotoxicity in PCB-exposed Chinese Hamster Ovary (CHO) cells pretreated with vitamin E

• Published in: Food and chemical toxicology Vol. 99; pp. 17 - 23
• Main Authors: Murati, Teuta, Šimić, Branimir, Pleadin, Jelka, Vukmirović, Maja, Miletić, Marina, Durgo, Ksenija, Kniewald, Jasna, Kmetič, Ivana
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2017
• Subjects: Animals; Antioxidants - pharmacology; Cell Survival - drug effects; CHO Cells; Cricetinae; Cricetulus; Cytotoxicity; Environmental Pollutants - toxicity; Lipid Peroxidation - drug effects; Ovary cells; PCB 153; PCB 77; Polychlorinated Biphenyls - toxicity; Reactive Oxygen Species - metabolism; Vitamin E; Vitamin E - pharmacology; Ovary cells; PCB 77; NR; PCB 153; CHO-K1; DCF; NFĸB; TCDD; Cytotoxicity; AhR; MDA; MTT; TOH; PCB; TB; BSA; DCFH-DA; TBA; TCA; Vitamin E; KB; ROS; IC
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2016.11.014

The aim of this study was to evaluate protective effects of vitamin E (50 -150 μM) in ovary cells upon cytotoxic effects induced by two structurally distinct PCB congeners - planar „dioxin-like” PCB 77 and non-planar di-ortho-substituted PCB 153 with an emphasis on identifying differences in the mechanism of vitamin E action depending on the structure of congeners. Application of three bioassays confirmed that PCBs decrease ovarian cell proliferation with slightly profound effects of PCB 77. PCB - induced ROS production and lipid peroxidation were significant for both congeners with also more noticeable effect for PCB 77. Vitamin E pre-incubation has improved viability of cells, reduced ROS formation and lipid peroxidation induced by PCBs' treatment. Preincubation with vitamin E was more effective when cells where treated with non-planar PCB 153. Altogether, vitamin E action was protective, congener specific and more effective when ovary cells were exposed to ortho-substituted PCB congener. [Display omitted] •PCB 77 and PCB 153 have shown significant cytotoxicity in ovary cells, with slightly profound effect of planar PCB 77.•Vitamin E has protective and congener specific effect on PCB-induced cytotoxicity and oxidative stress.•Protective role of vitamin E is more effective when ovary cells were exposed to ortho-substituted PCB congener.