Determination of debrisoquine and 4-hydroxydebrisoquine in urine by high-performance liquid chromatography with fluorescence detection after solid-phase extraction

A simple, selective and sensitive method has been developed to determine debrisoquine and 4-hydroxydebrisoquine in human urine. Separation of the analytes was obtained using a mobile phase of 0.1 M sodium dihydrogen phosphate-acetonitrile (87:13, v/v) and a muBondapak C18 column. The column effluent...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 11; no. 8; p. 745
Main Authors Bozkurt, A, Basci, N E, Isimer, A, Kayaalp, S O
Format Journal Article
LanguageEnglish
Published England 01.08.1993
Subjects
Acetonitriles - chemistry
Chromatography, High Pressure Liquid
Debrisoquin - analogs & derivatives
Debrisoquin - urine
Humans
Phosphates - chemistry
Polymorphism, Genetic
Sensitivity and Specificity
Spectrometry, Fluorescence
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Summary:A simple, selective and sensitive method has been developed to determine debrisoquine and 4-hydroxydebrisoquine in human urine. Separation of the analytes was obtained using a mobile phase of 0.1 M sodium dihydrogen phosphate-acetonitrile (87:13, v/v) and a muBondapak C18 column. The column effluent was monitored with fluorescence detection at 210 nm (ex) and 290 nm (em). Rapid sample preparation was achieved by solid-phase extraction columns (Bond Elut CBA, 3 ml capacity) which provided excellent recovery values for both compounds. The cost per sample using this approach could be minimized by column regeneration and re-use. The within-day and the day-to-day reproducibilities were less than 7% for both components. The method was shown to be suitable for the study of the debrisoquine-sparteine type genetic polymorphism in man.
ISSN:0731-7085
DOI:10.1016/0731-7085(93)80184-3