Ready display of antigenic peptides in a protein 'mimogen'
Given the dependence of much modern biology upon the use of antibodies as tools and reagents, their variability and the often associated lack-of-detail about function and identity creates experimental errors. Here we describe the proof-of-principle for a potentially general, versatile method for the...
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Published in | Chemical communications (Cambridge, England) Vol. 52; no. 14; pp. 3014 - 3017 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
18.02.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Given the dependence of much modern biology upon the use of antibodies as tools and reagents, their variability and the often associated lack-of-detail about function and identity creates experimental errors. Here we describe the proof-of-principle for a potentially general, versatile method for the display of antigens in a soluble yet standard format on a lateral protein scaffold that mimics normal epitopes in a protein antigen (a 'mimogen') and confirm their utility in phosphorylation-dependent recognition by specific antibodies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1359-7345 1364-548X |
DOI: | 10.1039/c5cc08892e |