Ready display of antigenic peptides in a protein 'mimogen'

Given the dependence of much modern biology upon the use of antibodies as tools and reagents, their variability and the often associated lack-of-detail about function and identity creates experimental errors. Here we describe the proof-of-principle for a potentially general, versatile method for the...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 52; no. 14; pp. 3014 - 3017
Main Authors Vallée, M Robert J, Schombs, Matthew W, Balaban, Zack J, Colyer, John, Davis, Benjamin G
Format Journal Article
LanguageEnglish
Published England 18.02.2016
Subjects
Antibodies
Antigens
Antigens - metabolism
Biology
Peptides - metabolism
Proteins
Reagents
Recognition
Utilities
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Summary:Given the dependence of much modern biology upon the use of antibodies as tools and reagents, their variability and the often associated lack-of-detail about function and identity creates experimental errors. Here we describe the proof-of-principle for a potentially general, versatile method for the display of antigens in a soluble yet standard format on a lateral protein scaffold that mimics normal epitopes in a protein antigen (a 'mimogen') and confirm their utility in phosphorylation-dependent recognition by specific antibodies.
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ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc08892e