Intracellular pH changes induced by exposure to weak acids and bases in submandibular cells of early postnatal rats

• Published in: Archives of oral biology Vol. 37; no. 9; pp. 699 - 703
• Main Authors: Seagrave, JC, Curry, M., Ramsey, R.Chaney, Martinez, J.R.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.09.1992; Elsevier Science
• Subjects: [formula omitted] antiport; Acetylcholine - pharmacology; Aging; Amiloride - pharmacology; Ammonium Chloride - pharmacology; Animals; Biological and medical sciences; Bumetanide - pharmacology; Butyrates - pharmacology; Butyric Acid; calcium; Cells, Cultured; Dentistry; development; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; intracellular pH; Ionomycin - pharmacology; Male; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus; neonates; Rats; Rats, Sprague-Dawley; submandibular gland; Submandibular Gland - cytology; Submandibular Gland - drug effects; Submandibular Gland - physiology; Substance P - pharmacology; Time Factors; Vertebrates: digestive system; development; calcium; intracellular pH; neonates; submandibular gland; Na + H + antiport; Rat; Digestive system; Weak base; Rodentia; Submandibular gland; Ion transport; Salivary gland; Postnatal development; Vertebrata; Mammalia; pH; Intracellular; Weak acid
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/0003-9969(92)90075-J

The intracellular pH of submandibular cells of adult, 1-day-old and 1-week-old rats was measured with the pH sensitive fluorimetric indicator SNARF-1, following shifts induced by exposure to a weak acid (sodium butyrate) or a weak base (NH 4Cl). The effects of several ion transport inhibitors and agonists on both the pH change and the rate of recovery were examined. The results indicate that, in most respects, the processes involved in pH regulation were similar in the neonatal and mature cells. However, cholinergic and peptidergic stimulation accelerated recovery from sodium butyrate-induced acidification more, and from NH 4Cl-induced alkalinization less, in cell preparations from 1-day-old animals.