Radioimmunoassay of somatostatin in human plasma

• Published in: Clinica chimica acta Vol. 133; no. 3; pp. 241 - 251
• Main Authors: Etzrodt, H., Rosenthal, J., Schröder, K.E., Pfeiffer, E.F.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 14.10.1983; Elsevier
• Subjects: Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Animals; Antibody Specificity; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Histocytochemistry; Humans; Immune Sera - immunology; Pancreas - cytology; Proteins; Rabbits - immunology; Radioimmunoassay - methods; Somatostatin - blood; Somatostatin - immunology; Assay; Human; Radioimmunoassay; Peptide hormone; Somatostatin; Blood; Blood plasma
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/0009-8981(83)90268-1

A radioimmunoassay procedure for determination of somatostatin immunoactivity in human plasma is described. Labelling of tyrosine-1-somatostatin is performed by chloramine T. Specific activity of the tracer is 1.34 mol iodine for 1 mol of somatostatin. The immunoreactivity is 85%. Antisera are produced in rabbits at a titre of 179 × 10 −12 mol somatostatin per ml antiserum. Human plasma is extracted by n-butanol/2 mol/1 acetic acid. Recovery rate is 85–105%. The intra-assay CV varies from 12% to 1.7%, the inter-assay CV from 16% to 7.5%, depending on the concentration of somatostatin.