Anti-inflammatory and toxicological evaluation of Moussonia deppeana (Schldl. & Cham) Hanst and Verbascoside as a main active metabolite

• Published in: Journal of ethnopharmacology Vol. 187; pp. 269 - 280
• Main Authors: Gutiérrez-Rebolledo, Gabriel Alfonso, Garduño-Siciliano, Leticia, García-Rodríguez, Rosa Virginia, Pérez-González, Mariana Zuleima, Chávez, María Isabel, Bah, Moustapha, Siordia-Reyes, Georgina Alicia, Chamorro-Cevallos, Germán Alberto, Jiménez-Arellanes, María Adelina
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.07.2016
• Subjects: Animals; Anti-inflammatory; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Anti-Inflammatory Agents - toxicity; Antioxidant; Antioxidants - chemistry; Antioxidants - pharmacology; Antioxidants - therapeutic use; Antioxidants - toxicity; Biphenyl Compounds - chemistry; Carrageenan; Edema - chemically induced; Edema - drug therapy; Female; Glucosides - analysis; Magnoliopsida; Male; Medicine, Traditional; Mexico; Mice, Inbred BALB C; Moussonia deppeana; Oleanolic Acid - analysis; Phenols - analysis; Phorbol Esters; Phytochemicals - analysis; Phytotherapy; Picrates - chemistry; Plant Components, Aerial; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Extracts - toxicity; Toxicity tests; Traditional medicine Meso and Southern America; Triterpenes - analysis; Ursolic Acid; Verbascoside; Toxicity tests; Traditional medicine Meso and Southern America; Moussonia deppeana; Verbascoside; Antioxidant; Anti-inflammatory
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2016.04.033

Moussonia deppeana, known as Tlachichinole, is a Mexican medicinal plant used for treatment of inflammatory diseases, influenza, diarrhea, gastrointestinal disorders and arthritis. In this paper the antioxidant and anti-inflammatory activities as well as the acute and sub-acute toxicological effects were evaluated for the ethanolic extract from aerial parts of M. deppeana, also its phytochemical analysis is described. Phytochemical analysis and compound isolation were performed with thin layer chromatography. The chemical identification of the main compound was performed by 1H NMR (COSY, NOESY, HSQC and HMBC) spectra. In vitro antioxidant capacity and total phenolic content for the ethanolic extract and its primary fractions was determined by DPPH and Folin-Ciocalteu reagent. Acute and subacute toxicity tests were evaluated on Balb/C mice. Finally acute anti-inflammatory evaluation was tested for a local (TPA) and systemic (carrageenan) murine model. The main compound isolated from the ethanolic extract of M. deppeana was Verbascoside, which was isolated from F3 and was identified by 1H NMR and COSY data. Furthermore oleanolic and ursolic acids were isolated from primary fractions F1 and F2. Ethanolic extract showed IC50 = 6.71mg/mL for DPPH test and 664.12µg QE/mL for the total phenolic content. The LD50 value was >2g/kg by i.g. route in male and female mice. Sub-acute administration (28 days) of the ethanolic extract (1g/kg) did not cause lethality or alter any hematological and biochemical parameters, in addition, histological analysis of the major organs exhibited no structural changes. Anti-inflammatory activity of the ethanolic extract showed an ED50 = 1.5mg/ear and 450mg/kg for TPA and carrageenan test, respectively. Primary fractions generated moderate local and systemic anti-inflammatory activity. The ethanolic extract from the aerial parts of M. deppeana did not cause any lethality or adverse effect in either of the acute and sub-acute toxicity tests. This exhibited an important local and systemic anti-inflammatory activity and also moderate antioxidant capacity. Moreover, the primary fraction F2 was more active for the TPA model while the primary fraction F3 was most active in the carrageenan model in vivo. The main compound isolated from F3 was verbascoside; on the other hand also ursolic and oleanolic acids were isolated from F1 and F2. [Display omitted]