Dendrobine enhances bovine oocyte maturation and subsequent embryonic development and quality

• Published in: Theriogenology Vol. 203; pp. 53 - 60
• Main Authors: Zhao, Yuhan, E, Zhiqiang, Jiao, Anhui, Sun, Zhaoyang, Zhang, Hongbo, Wang, Haijun, Fang, Nanzhu, Gao, Qingshan, Jin, Qingguo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2023
• Subjects: Animals; Apoptosis; Blastocyst - physiology; Bovine oocytes; Cattle; Dendrobium nobile extract; Embryonic Development; In Vitro Oocyte Maturation Techniques - methods; In Vitro Oocyte Maturation Techniques - veterinary; Oocytes - physiology; Oxidative stress; Reactive Oxygen Species - metabolism; Embryonic development; Oxidative stress; Bovine oocytes; Dendrobium nobile extract; Apoptosis
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2023.03.015

Strategies for improving the quality of oocytes have important theoretical and practical significance for increasing the efficiency of livestock breeding. In this respect, the accumulation of reactive oxygen species (ROS) is a major factor affecting the development of oocytes and embryos. This study investigated the effects of Dendrobium nobile extract (DNE) on the in vitro maturation of bovine oocytes and embryonic development after IVF. DNE is an extract from Dendrobium rhizomes that contains alkaloids with anti-inflammatory, anti-cancer and anti-ageing functions. Various concentrations of DNE (0, 5, 10, 20 and 50 μmol/L) were added during oocyte maturation in vitro, and we found that 10 μmol/L of DNE remarkably increased the oocyte maturation rate, the subsequent blastocyst formation rate and embryo quality. Further, we found that DNE treatment decreased the frequency of spindle/chromosome defects and ROS and increased the oocyte glutathione and mitochondrial membrane potential in oocytes. Moreover, DNE upregulated the expression of oxidative stress-related genes (Sirt1, Sirt2, Sirt3 and Sod1) in oocytes and apoptosis-related genes (Caspase-3, Caspase-4, Bax, Bcl-xl and Survivin) in blastocysts. These results suggest that DNE supplementation can promote oocyte maturation and subsequent embryonic development by regulating redox reactions and inhibiting embryonic apoptosis. •DNE promotes oocyte maturation and embryo development.•DNE decreases ROS and increases MMP.•DNE has an inhibitory effect on embryonic apoptosis.