Complete Purging of Ewing Sarcoma Metastases from Human Ovarian Cortex Tissue Fragments by Inhibiting the mTORC1 Signaling Pathway

Restoration of fertility by autologous transplantation of ovarian cortex tissue in former cancer patients may lead to the reintroduction of malignancy via the graft. Pharmacological ex vivo purging of ovarian cortex fragments prior to autotransplantation may reduce the risk of reseeding the cancer....

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Published inJournal of clinical medicine Vol. 10; no. 19; p. 4362
Main Authors Peek, Ronald, Eijkenboom, Lotte L., Braat, Didi D. M., Beerendonk, Catharina C. M.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 24.09.2021
MDPI
Subjects
Breast cancer
Cancer therapies
Cell growth
Chemotherapy
Clinical medicine
Cloning
Cryopreservation
Disease transmission
Ewings sarcoma
Fertility
Kinases
Leukemia
Metastasis
Oophorectomy
Ovaries
Patients
Signal transduction
Targeted cancer therapy
Transcription factors
United States > US
Switzerland
Netherlands
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Summary:Restoration of fertility by autologous transplantation of ovarian cortex tissue in former cancer patients may lead to the reintroduction of malignancy via the graft. Pharmacological ex vivo purging of ovarian cortex fragments prior to autotransplantation may reduce the risk of reseeding the cancer. In this study we have investigated the capacity of Everolimus (EVE), an inhibitor of the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway, to eradicate Ewing’s sarcoma (ES) from ovarian tissue by a short-term ex vivo treatment. Exposure of experimentally induced ES tumor foci in ovarian tissue to EVE for 24 h completely eliminated the malignant cells without detrimental effects on follicle morphology, survival or early folliculogenesis. This indicates that effective purging of ovarian cortex tissue from contaminating ES tumor foci is possible by short-term exposure to EVE.
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ISSN:2077-0383
2077-0383
DOI:10.3390/jcm10194362