Different regulation of hepatic peroxisomal β-oxidation activity in rats treated with clofibrate and partially hydrogenated marine oil

• Published in: Biochemical and biophysical research communications Vol. 166; no. 2; pp. 780 - 786
• Main Authors: Horie, Shuichi, Suga, Tetsuya
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.01.1990; Elsevier
• Subjects: Acyl-CoA Oxidase; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Clofibrate - pharmacology; Fish Oils - pharmacology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic - drug effects; Lipids; Liver - metabolism; Male; Microbodies - enzymology; Other biological molecules; Oxidoreductases - genetics; Rats; Rats, Inbred Strains; RNA, Messenger - genetics; SDS-PAGE; DEHP; AO; PHMO; Vertebrata; Mammalia; Messenger RNA; Rat; Induction; Liver; Rodentia; Peroxisome; Marine oil; Antilipemic agent
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(90)90877-P

Total RNAs from the livers of rats treated with clofibrate and partially hydrogenated marine oil (PHMO) wer translated in a reticulocyte-lysate cell-free protein-synthesizing system. In clofibrate-treated rats, mRNA activity for acyl-CoA oxidase (AO), the rate-limiting enzyme of the peroxisomal β-oxidation system, was increased markedly compared with the control, whereas the increase was less than 2-fold in PHMO-treated rats. When rats were treated with both clofibrate and PHMO in vivo , an additional increase in the hepatic AO activity was observed compared with either treatment alone, suggesting that increases in the activities of peroxisomal β-oxidation in the rats treated with clofibrate and PHMO are based on two distinct mechanisms.