Droplet vitrification versus straw cryopreservation for spermatozoa banking in Persian sturgeon (Acipenser persicus) from metabolite point of view

• Published in: Theriogenology Vol. 129; pp. 110 - 115
• Main Authors: Abed-Elmdoust, Amirreza, Rahimi, Ruhollah, Farahmand, Hamid, Amiri, Bagher Mojazi, Mirvaghefi, Alireza, Rafiee, Gholamreza
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.04.2019
• Subjects: 1H NMR spectroscopy; Animals; Cryopreservation; Cryopreservation - methods; Cryopreservation - veterinary; Fishes; Male; Nuclear Magnetic Resonance, Biomolecular; Persian sturgeon; Semen Preservation - methods; Semen Preservation - veterinary; Spermatozoa; Spermatozoa - metabolism; Spermatozoa - physiology; Vitrification; Spermatozoa; 1H NMR spectroscopy; Cryopreservation; Persian sturgeon; Vitrification; H NMR spectroscopy
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2019.02.031

Persian sturgeon (Acipenser persicus), a commercially valuable and critically endangered fish species has been suffering considerable declines in populations in the nature due to over-fishing, habitat destruction and marine pollution during past decades. Since there were no achievements in artificial reproduction programs, genetic resource banking such as gametes and embryo cryopreservation can be a good strategy however, reported resulting gamete qualities were considerably low. In the present study, the metabolome content of Persian sturgeon spermatozoa was investigated during common straw cryopreservation and novel droplet vitrification by the use of 1H NMR (Nuclear Magnetic Resonance) spectroscopy. Univariate (ANOVA) and multivariate (PCA) analysis showed significant differences in the metabolic profiles between cryopreserved and fresh spermatozoa samples. Adenine, creatine, creatine phosphate, glucose, guanidoacetate, lactate, N, N-dimethylglycine, and glycine levels showed no significant differences between these two cryopreservation techniques suggesting these metabolites and their corresponding enzymes and chemical pathways are so vulnerable to the temperature changes and even higher cooling rate in droplet vitrification could not conserve them. However, significant differences were found in acetate, creatinine, betaine, β-alanine and trimethylamine N-oxide suggesting better efficiency of droplet vitrification in protection of some metabolites associated to spermatozoa energetics, redox balance and hypoxia compensation compared to straw cryopreservation. •Spermatozoa quality indexes and metabolites of two commonly used cryopreservation techniques were compared.•All detected and quantified metabolites in cryopreserved spermatozoa had significant difference compared to the fresh spermatozoa.•Metabolites, corresponding enzymes and chemical pathways that are more vulnerable to the temperature changes are introduced.•Relative biochemical pathways and enzymes involved are discussed.