Changes in cellular secretory processing during baculovirus infection

• Published in: Biotechnology letters Vol. 27; no. 14; pp. 1041 - 1045
• Main Authors: Yun, Eun-Young, Goo, Tae-Won, Kim, Sung-Wan, Choi, Kwang-Ho, Hwang, Jae-Sam, Kang, Seok-Woo, Kwon, O-Yu
• Format: Journal Article
• Language: English
• Published: Dordrecht Kluwer Academic Publishers 01.07.2005; Springer; Springer Nature B.V
• Subjects: Animals; Baculoviridae - growth & development; Biological and medical sciences; Biotechnology; Bombyx - enzymology; Bombyx - virology; Bombyx mori; calnexin; Calreticulin - metabolism; endoplasmic reticulum; Endoplasmic Reticulum - enzymology; Endoplasmic Reticulum - metabolism; Fundamental and applied biological sciences. Psychology; green fluorescent protein; Insect Proteins - metabolism; molecular chaperones; protein disulfide-isomerase; Protein Disulfide-Isomerases - metabolism; Proteins; Secretion; Chaperone; Insecta; Sf9 insect cell line; Gene expression; Virus; Infection; Cell line; Baculoviridae; Arthropoda; Invertebrata; Endoplasmic reticulum; Vector; baculovirus expression vector system; endoplasmic reticulum molecular chaperone
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-005-8108-1

In the baculovirus expression vector system (BEVS), secretory green fluorescent protein (sGFP) transcripts were expressed from day 2 to day 5 post-infection (p.i.), while transcripts of the endoplasmic reticulum (ER) molecular chaperone Bombyx mori protein disulfide isomerase (bPDI) were measurable in mock cells and in cells at day 1 p.i. The GFP was expressed from day 3 to day 5 p.i. whereas the levels of two ER chaperone proteins, bPDI and calnexin, decreased from day 3 p.i. and were not detected from day 4 p.i. These findings suggest that the rate-limited expression of ER molecular chaperones is strongly associated with the maximal expression of exogenous proteins in BEVS.