Supplementation of lycopene in maturation media improves bovine embryo quality in vitro

This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-sta...

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Published inTheriogenology Vol. 103; pp. 173 - 184
Main Authors Chowdhury, M.M.R., Choi, Byung-Hyun, Khan, Imran, Lee, Kyeong-Lim, Mesalam, Ayman, Song, Seok-Hwan, Xu, Lianguang, Joo, Myeong-Don, Afrin, Fahmida, Kong, Il-Keun
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.2017
Subjects
Animals
Apoptosis - drug effects
Blastocyst - cytology
Blastocyst - physiology
Bovine
Carotenoids - chemistry
Carotenoids - pharmacology
Cattle - embryology
Culture Media
Cumulus Cells
Embryo culture
Embryo Culture Techniques - veterinary
Embryonic Development - drug effects
Fertilization in Vitro
Free radicals
In Vitro Oocyte Maturation Techniques
Lycopene
Molecular Structure
Oocytes - physiology
Oxidative stress
Reactive Oxygen Species
RNA, Messenger
Oxidative stress
Free radicals
Bovine
Embryo culture
Lycopene
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Summary:This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0.05), Immunocytochemistry confirmed that diminished protein expression of nuclear factor kappa B (NFκB), cyclooxygenase-2 (COX2), and 8-oxoguanine (indicated by ROS) and relative mRNA expression of the Caspase-3, NFκB, COX2, iNOS and BCL2-associated X (BAX) was significantly (P < 0.05) lower whereas the anti-apoptotic gene BCL2 was significantly (P < 0.05) higher in the 0.2 μM lycopene-supplemented group than the control. In conclusion, lycopene improves blastocyst quality by overcoming unfavorable conditions in in vitro culture systems.
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ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2017.08.003