Early embryo loss, morphology, and effect of previous immunization against androstenedione in the ewe

• Published in: Theriogenology Vol. 86; no. 5; pp. 1285 - 1293
• Main Authors: O'Connell, Anne R., Demmers, Kristina J., Smaill, Bronwyn, Reader, Karen L., Juengel, Jennifer L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2016
• Subjects: Androstenedione; Androstenedione - immunology; Animals; Embryo morphology; Embryo mortality; Female; Immunization; Ovulation; Ovulation - physiology; Pregnancy; Pregnancy Rate; Sheep; Sheep - embryology; Tissue and Organ Harvesting; Trophoblast; Sheep; Trophoblast; Embryo morphology; Androstenedione; Ovulation; Embryo mortality
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2016.04.069

In a naturally mated cycle, ova and viable embryo number as well as embryo size were assessed on Day 4, 10, 14, 18, and 30 of gestation in Romney ewes (n = 38–44 per gestational group). For Days 4–18 of gestation, embryos were recovered by flushing the reproductive tract after slaughtering of the ewe. Ovulation rate was determined by counting the number of corpora lutea present on both ovaries. For the Day 30 group, number of ovulations was measured by laparoscopic examination of the ovaries at Day 9–12 of the cycle, and number of embryos present was determined by ultrasound examination at approximately Day 30 of pregnancy. Most of embryo loss occurred before Day 14 of gestation with 6% loss before Day 4, and 12% loss between Day 4 and 14 of gestation. A similar proportion of viable embryos per number of ova ovulated were recovered on Day 14 and 18 (82%) and Day 30 (81%) of gestation. Fertilization failure was estimated at 1%. Conceptus and embryo size was most variable on Day 14, representing a period of rapid growth (conceptus length ± standard deviation); Day 4 (169 ± 8 μm), Day 10 (379 ± 93 μm), Day 14 (23 ± 32 mm), Day 18 (embryo length ± standard deviation; 5.0 ± 0.7 mm). Vaccination with commercially available fertility vaccines targeting androstenedione (Androvax and Ovastim) in previous seasons resulted in reduced conceptus size compared with controls. However, no difference in the proportion of viable embryos was observed between treatments, signifying maternal tolerance for considerable variation at this stage of development. Furthermore, the finding that most of loss occurs within the first 14 days of gestation highlights the importance of both oocyte quality and the uterine environment for the embryo to successfully overcome the challenges leading up to and including pregnancy recognition in the ewe.