A non-chromatographic radioimmunoassay for 3-oxo desogestrel

• Published in: Journal of steroid biochemistry Vol. 22; no. 1; pp. 111 - 113
• Main Authors: Shaw, M.A., Back, D.J., Cowie, Anne M., Orme, M.C.L'E.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.1985; New York, NY Pergamon
• Subjects: 3-oxo desogestrel, 13β-ethyl-17-hydroxy-11-methylene-18,19-dinor-pregn-4-en-20-yn-3-one; 3-oxo, 5α dihydro desogestrel, 13β-ethyl-11-methylene-18,19-dinor-5α,pregn-20-yn-3-one; 3α-hydroxy 5α dihydro desogestrel, 13β-ethyl-11-methylene-18,19-dinor-5α,pregn-20-yne-3α,17-diol; 3β-hydroxy desogestrel, 13β-ethyl-11-methylene-18,19-dinor-pregn-4-en-20-yne-3β,17-diol; 3β-hydroxy-5α dihydrodesogestrel, 13β-ethyl-11-methylene-18,19-dinor-5α,pregn-20-yne-3β,17-diol; Adult; Biological and medical sciences; Desogestrel; ethynyloestradiol, 17α-ethynyl-1,3,5 -estratriene-3,17β-diol; General pharmacology; Half-Life; Humans; Kinetics; levonorgestrel, 13β-ethyl-17-hydroxy-18,19-dinor-pregn-4-en-20-yn-3-one; lynestrenol, 19-nor-pregn-4-en-20-yn-17-ol; Male; Medical sciences; norethisterone, 17-hydroxy-19-nor-pregn-4-en-20-yn-3-one; Norpregnenes - blood; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions; Pharmacology. Drug treatments; Radioimmunoassay; The following steroid names are used in this paper:desogestrel, 13β-ethyl-11-methylene-18,19-dinor-pregn-4-en-20-yn-17-ol; 3β-hydroxy desogestrel, 13β-ethyl-11-methylene-18,19-dinor-pregn-4-en-20-yne-3β,17-diol; norethisterone, 17-hydroxy-19-nor-pregn-4-en-20-yn-3-one; levonorgestrel, 13β-ethyl-17-hydroxy-18,19-dinor-pregn-4-en-20-yn-3-one; 3α-hydroxy 5α dihydro desogestrel, 13β-ethyl-11-methylene-18,19-dinor-5α,pregn-20-yne-3α,17-diol; The following steroid names are used in this paper:desogestrel, 13β-ethyl-11-methylene-18,19-dinor-pregn-4-en-20-yn-17-ol; 3-oxo desogestrel, 13β-ethyl-17-hydroxy-11-methylene-18,19-dinor-pregn-4-en-20-yn-3-one; 3-oxo, 5α dihydro desogestrel, 13β-ethyl-11-methylene-18,19-dinor-5α,pregn-20-yn-3-one; ethynyloestradiol, 17α-ethynyl-1,3,5 -estratriene-3,17β-diol; lynestrenol, 19-nor-pregn-4-en-20-yn-17-ol; 3β-hydroxy-5α dihydrodesogestrel, 13β-ethyl-11-methylene-18,19-dinor-5α,pregn-20-yne-3β,17-diol; Assay; Synthetic product; Radioimmunoassay; Technique; Sex steroid hormone; Pharmacokinetics; Progestagen
• ISSN: 0022-4731
• DOI: 10.1016/0022-4731(85)90149-9

A non-chromatographic radioimmunoassay for 3-oxo desogestrel (13β-ethyl-17-hydroxy-11-methylene-18,19-dinor-pregn-4-en-20-yn-3-one), the biologically-active metabolite of desogestrel (13β -ethyl-11-methylene-18,19-dinor-pregn-4-en-20-yn-17-ol), has been developed to facilitate studies of the pharmacokinetics of this steroid. The method uses an antiserum raised against levonorgestrel (13β-ethyl-17-hydroxy-18,19-dinor-pregn-4-en-20-yn-3-one). None of the steroids tested which showed significant cross-reactions are believed to be present in plasma after ingestion of desogestrel; furthermore, dilutions of standards and unknowns gave parallel responses in the assay. Intra- and inter-assay coefficients of variation were 12.9 and 11.8% respectively. The sensitivity of the assay was approx 0.02 ng/ml. The peak concentrations of 3-oxo desogestrel after a 150 μg dose of desogestrel in three subjects were between 0.48–0.71 ng/ml, and in two subjects 3-oxo desogestrel was still detectable 24 h after dosing.