Growth and Metabolite Production in Chlorella sp.: Analysis of Cultivation System and Nutrient Reduction
Microalgae are a sustainable source for many bioproduct syntheses. This study shows how different cultivation systems and nutrient concentrations affect Chlorella sp. development. The cultivation was performed in open and closed lab-scale systems. A Tukey test was used to evaluate the significant di...
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Published in | Bioenergy research Vol. 16; no. 3; pp. 1829 - 1840 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.09.2023
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Microalgae are a sustainable source for many bioproduct syntheses. This study shows how different cultivation systems and nutrient concentrations affect
Chlorella
sp. development. The cultivation was performed in open and closed lab-scale systems. A Tukey test was used to evaluate the significant differences between the microalgae growth and composition under the two systems analyzed. The effect of nitrogen and phosphorus concentration was analyzed with a 2
2
full factorial design. Lipids, carbohydrates, proteins, and pigments were extracted and quantified. The highest specific growth rate (0.52 1/day) was obtained in the open pond, with a cell density of 5.06 × 10
7
cells/mL and a biomass concentration of 1.30 mg/mL. In this condition, the higher concentration of pigments was obtained: 13 μg/mg of chlorophyll-a, 15 μg/mg of chlorophyll-b, and 2 μg/mg of carotenoids. An expressive increase in the lipid (from 17 to 35%) was obtained in low nitrogen and high phosphorus. Protein percentage increases with both nutrients at a higher level, reaching up to 60%. The percentage of carbohydrates decreases from 32 to 13% with phosphorus reduction. Thus, this study points out the conditions that benefit the accumulation of each
Chlorella
sp. metabolite, contributing to the decision-making for the cultivation and applications of this microalgae. |
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ISSN: | 1939-1234 1939-1242 |
DOI: | 10.1007/s12155-022-10532-z |