Time changes in Na +, K +-ATPase, Mg ++-ATPase, and acetylcholinesterase activities in the rat cerebrum and cerebellum caused by stress

• Published in: Pharmacology, biochemistry and behavior Vol. 44; no. 2; pp. 339 - 342
• Main Authors: Tsakiris, Stylianos, Kontopoulos, Alexander N.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.1993; Elsevier Science
• Subjects: Acetylcholine - physiology; Acetylcholinesterase; Acetylcholinesterase - metabolism; Animals; Biological and medical sciences; Brain - enzymology; Ca(2+) Mg(2+)-ATPase - metabolism; Cerebellum - enzymology; Cold and immobilization stress; Corticosterone - metabolism; Cyclic AMP - physiology; Fundamental and applied biological sciences. Psychology; Male; Mg ++-ATPase; Na +,K +-ATPase; Neurons - enzymology; Norepinephrine - physiology; Personality. Affectivity; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rat cerebrum and cerebellum; Rats; Rats, Wistar; Sodium-Potassium-Exchanging ATPase - metabolism; Stress; Stress, Physiological - enzymology; Sympathetic Nervous System - physiology; Time Factors; Time-related changes; Cold and immobilization stress; Time-related changes; Na +,K +-ATPase; Acetylcholinesterase; Rat cerebrum and cerebellum; Mg ++-ATPase; Cerebellum; Cold; Rat; Enzyme; Rodentia; Central nervous system; Stress; Vertebrata; Mammalia; Enzymatic activity; Animal; Na,K-ATPase; Restraint; Mg-ATPase; Brain (vertebrata)
• ISSN: 0091-3057; 1873-5177
• DOI: 10.1016/0091-3057(93)90471-5

Na +,K +-ATPase, Mg ++-ATPase, and acetylcholinesterase activities were determined in homogenated rat cerebrum and cerebellum in unstressed animals (control) and exposed to cold and immobilization for 45–180 min. Na +,K +-ATPase and Mg ++-ATPase activities were not affected within the first 80 min of stress, while they were increased about 50–70% after 120–180 min, where the maximum enzyme stimulation was observed. However, acetylcholinesterase activity was increased considerably by less than 45 min of stress and reached a plateau in 80–180 min to a higher value in the cerebrum ( ≊100%) than in the cerebellum ( ≊40%) related to the control. Our results suggest that: a) The stress used can stimulate acetylcholinesterase in a different way and more quickly than Na +,K +-ATPase and Mg ++-ATPase; b) acetylcholinesterase in the cerebellum is stimulated to a lower level than in the cerebrum by stress, probably because of the presence of a relatively small cerebellar cholinergic innervation.