Optical fiber-based photomechanical gene transfer system for in vivo application

We developed an optical-fiber-based photomechanical gene transfer system for endoscopic or catheter-based application. A fiber tip with a laser-absorbing film covered with a transparent plastic disk for plasma confinement was attached to a quartz fiber; the film was irradiated with nanosecond laser...

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Bibliographic Details
Published inOptics letters Vol. 36; no. 23; p. 4545
Main Authors Sato, Shunichi, Ando, Takahiro, Obara, Minoru
Format Journal Article
LanguageEnglish
Published United States 01.12.2011
Subjects
Animals
Gene Transfer Techniques - instrumentation
Genes, Reporter
Green Fluorescent Proteins - genetics
Lasers, Solid-State - therapeutic use
Luciferases - genetics
Male
Optical Fibers
Optical Phenomena
Pressure
Rats
Rats, Sprague-Dawley
Skin - metabolism
Skin - radiation effects
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Summary:We developed an optical-fiber-based photomechanical gene transfer system for endoscopic or catheter-based application. A fiber tip with a laser-absorbing film covered with a transparent plastic disk for plasma confinement was attached to a quartz fiber; the film was irradiated with nanosecond laser pulses transmitted through the fiber to generate photomechanical waves (PMWs). Characteristics of PMWs emitted from the fiber tip were examined to confirm the necessary conditions for gene transfer. We then attempted to transfer reporter genes to the rat skin as a test tissue in vivo with the fiber system, and the results showed significantly high protein levels and spatially selective pinpoint gene expressions in the tissue.
ISSN:1539-4794
DOI:10.1364/ol.36.004545