Human plasma contains low molecular weight factors which stimulate active sodium transport in erythrocytes

• Published in: Clinica chimica acta Vol. 179; no. 2; pp. 133 - 142
• Main Authors: Tepper, T., Huisman, R.M., Jilderda, J.F., van der Hem, G.K., de Zeeuw, D.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 15.02.1989; Elsevier
• Subjects: Adult; Biological and medical sciences; Biological Transport, Active; Blood Glucose - analysis; Cell physiology; Electrolytes - blood; Erythrocyte; Erythrocytes - metabolism; Fundamental and applied biological sciences. Psychology; Furosemide; Furosemide - pharmacology; Humans; Kinetics; Membrane and intracellular transports; Molecular and cellular biology; Molecular Weight; Ouabain; Ouabain - pharmacology; Plasma sodium transport; Sodium - blood; Sodium - metabolism; Erythrocyte; Plasma sodium transport; Furosemide; Ouabain; Human; Red blood cell; Ion transport; Sodium; Blood plasma
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/0009-8981(89)90159-9

The aim of this study was to establish whether differences in sodium efflux rate constants ( K e ) in human erythrocytes occur when artificial media are compared with plasma. Using a 22Na tracer method, a mean total K e of 0.49 ± 0.10 h −1 and significantly ( p < 0.05) lower K e values in Hanks' solution (0.43 ± 0.08 h −1) and Basic Salt Solution (0.37 ± 0.07 h −1) were observed. Exhaustive dialysis of plasma against Hanks' solution over a membrane with relative molecular mass cut-off of 1000 Da resulted in a decrease of the plasma total K e value to that measured in Hanks' solution. After equilibrium dialysis of plasma against Hanks' solution a decrease of total K e was found in plasma and an increase of the K e in Hanks' solution was measured. The data suggest the presence of an excess of dialyzable, active sodium transport stimulating plasma factor(s) with relative molecular mass below 1000 Da.