Native fluorescence of the cervix uteri as a marker for dysplasia and invasive carcinoma

• Published in: European journal of obstetrics & gynecology and reproductive biology Vol. 31; no. 3; pp. 249 - 253
• Main Authors: Lohmann, Wolfgang, Muβmann, Jörg, Lohmann, Chris, Künzel, Wolfgang
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.06.1989; Elsevier
• Subjects: Biological and medical sciences; Carcinoma; Cervix uteri; Cervix Uteri - pathology; Dysplasia; Female; Female genital diseases; Fluorescence; Gynecology. Andrology. Obstetrics; Humans; Medical sciences; NAD - metabolism; NADH; Native fluorescence; Neoplasm Staging; Tumors; Uterine Cervical Dysplasia - pathology; Uterine Cervical Neoplasms - pathology; Dysplasia; Cervix uteri; Carcinoma; NADH; Native fluorescence; Human; NADPH; Carcinoma in situ; Fluorescence; Tumor; Tumoral marker; Uterine cervix; Female genital diseases; Chromophore
• ISSN: 0301-2115; 1872-7654
• DOI: 10.1016/0028-2243(89)90160-3

A non-invasive and non-destructive fluorescence technique developed recently for an in situ detection of melanomas has been applied for determining in vitro dysplasia and invasive carcinomas in the cervix uteri. The cervices uteri exhibit a fluorescence band with a peak at about 475 nm if excited with 365 nm. The fluorescence intensity increases concomitantly with the degree of dysplasia, ranging from 30 counts/100 ms (healthy) to ~ 200 counts per 100 ms (CIN 3). At the rim of a malignancy, the intensity is 250 counts/100 ms and higher and decreases towards the healthy region. In the tumor region, the intensity is about zero or very small, at the most. The naturally occurring chromophore being responsible for the fluorescence observed seems to be NADH.