Love wave biosensor for real-time detection of okadaic acid as DSP phycotoxin

Love wave acoustic delay lines were used as sensors for okadaic acid immuno-detection through immobilized specific antibodies. Anti-okadaic acid antibodies were grafted onto the sensor surface. Antibodies, saturating agent, and target species, okadaic acid (test line) or an irrelevant peptide (contr...

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Bibliographic Details
Published inProcedia Engineering Vol. 5; pp. 831 - 834
Main Authors Fournel, F., Baco, E., Mamani-Matsuda, M., Degueil, M., Bennetau, B., Moynet, D., Mossalayi, D., Vellutini, L., Pillot, J.-P., Dejous, C., Rebiere, D.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Elsevier Ltd 01.01.2010
Elsevier
Subjects
Acoustic wave
Biodetection
Engineering Sciences
Micro and nanotechnologies
Microelectronics
Microfluidic
Phycotoxin
Biodetection
Phycotoxin
Acoustic wave
Microfluidic
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Summary:Love wave acoustic delay lines were used as sensors for okadaic acid immuno-detection through immobilized specific antibodies. Anti-okadaic acid antibodies were grafted onto the sensor surface. Antibodies, saturating agent, and target species, okadaic acid (test line) or an irrelevant peptide (control line), were injected through an optimized microfluidic chip. Similarly to sandwich ELISA, polyclonal antibodies were then injected a second time to bind to the acid. They led to frequency shifts three times superior with okadaic acid than with the peptide.
ISSN:1877-7058
1877-7058
DOI:10.1016/j.proeng.2010.09.237