APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase ( Pf LDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL −1 ) and could rel...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 51; no. 22; pp. 4697 - 4700
Main Authors Dirkzwager, Roderick M., Kinghorn, Andrew B., Richards, Jack S., Tanner, Julian A.
Format Journal Article
LanguageEnglish
Published England 18.03.2015
Subjects
Animals
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Biomarkers
Biomarkers - blood
Biomarkers - metabolism
Blood
blood sampling
chemical compounds
chemical reactions
Colorimetry
detection limit
Diagnosis
Diagnostic systems
disease diagnosis
Enzymes
Erythrocytes - metabolism
Erythrocytes - parasitology
Humans
lactate dehydrogenase
Lactate Dehydrogenases - blood
Lactate Dehydrogenases - isolation & purification
Lactate Dehydrogenases - metabolism
Malaria
Malaria - diagnosis
Malaria - parasitology
Malaria - pathology
oligonucleotides
Plasmodium
Plasmodium falciparum
Plasmodium falciparum - enzymology
Rats
Robustness
Sensitivity and Specificity
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Summary:We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase ( Pf LDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL −1 ) and could reliably diagnose malaria in clinical blood samples.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/C5CC00438A