Insulin gene expression pattern in rat pancreas during the estrous cycle

Sex steroid hormones influence insulin homeostasis and glucose metabolism, estradiol (E 2) and progesterone (P 4) induce changes in both fasting and postprandial insulinemia in rodents, however, insulin gene expression during estrous cycle is unknown. The aim of the present study was to determine an...

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Published inLife sciences (1973) Vol. 68; no. 26; pp. 2979 - 2985
Main Authors Morimoto, Sumiko, Cerbón, Marco A., Alvarez-Alvarez, Aimeé, Romero-Navarro, Guillermo, Dı́az-Sánchez, Vicente
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 18.05.2001
Subjects
Animals
Estradiol - blood
Estrous cycle
Estrus - physiology
Female
Gene Expression Regulation - physiology
Glucose - metabolism
Homeostasis
Insulin
Insulin - blood
Insulin - genetics
Insulin expression
Islets of Langerhans - physiology
Progesterone - blood
Rat pancreas
Rats
Rats, Wistar
RNA, Messenger - analysis
Sex hormones
Testosterone - blood
Transcription, Genetic
Sex hormones
Estrous cycle
Insulin expression
Insulin
Rat pancreas
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Summary:Sex steroid hormones influence insulin homeostasis and glucose metabolism, estradiol (E 2) and progesterone (P 4) induce changes in both fasting and postprandial insulinemia in rodents, however, insulin gene expression during estrous cycle is unknown. The aim of the present study was to determine an insulin gene expression pattern during the estrous cycle in the rat. Groups of 6 adult rats in each day of the estrous cycle were used. Serum P 4, E 2, testosterone (T) and insulin concentrations were determined by radioimmunoassay (RIA). A Northern blot analysis was performed to assess insulin gene expression in pancreatic tissue. We found a marked variation in insulin gene expression during the estrous cycle. The highest insulin expression was observed during the proestrus day. Interestingly, E 2 and P 4 but not T levels were correlated with changes in insulin mRNA content. The variations in serum insulin during the cycle were correlated with its mRNA content in pancreas. The overall results showed variations in serum insulin and insulin gene expression during estrous cycle of the rat that correlated with circulating E 2 and P 4 levels.
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ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(01)01100-6