Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients

The lack of an adequate system for the in vitro cultivation of Cryptosporidium spp. has forced researchers to work on infected feces or tissues. Molecular and immunological analyses of Cryptosporidium stages must be preceded by complex preparatory steps involving the concentration, storage, purifica...

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Bibliographic Details
Published inThe American journal of tropical medicine and hygiene Vol. 36; no. 3; p. 505
Main Authors Kilani, R T, Sekla, L
Format Journal Article
LanguageEnglish
Published United States 01.05.1987
Subjects
Animals
Centrifugation, Density Gradient
Cesium
Chlorides
Coccidia - isolation & purification
Cryptosporidium - isolation & purification
Povidone
Silicon Dioxide
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Summary:The lack of an adequate system for the in vitro cultivation of Cryptosporidium spp. has forced researchers to work on infected feces or tissues. Molecular and immunological analyses of Cryptosporidium stages must be preceded by complex preparatory steps involving the concentration, storage, purification, excystation of oocysts, and purification of sporozoites. This paper describes two new procedures for the purification of Cryptosporidium. The first, consisting of pretreatment of oocysts with sodium hypochlorite followed by concentration using a Percoll gradient, is suitable for nucleic acid analyses. The second, a concentration of untreated oocysts using a Cesium chloride gradient, is suitable for biochemical and immunological studies, but requires "fresh" oocysts.
ISSN:0002-9637
DOI:10.4269/ajtmh.1987.36.505