Infrared spectroscopy of retinoids

• Published in: Analytical biochemistry Vol. 133; no. 2; pp. 314 - 321
• Main Authors: Rockley, Natalie L., Halley, Bruce A., Rockley, Mark G., Nelson, Eldon C.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1983; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemical Phenomena; Chemistry; Coenzymes, vitamins; Fourier Analysis; Fundamental and applied biological sciences. Psychology; infrared; Other biological molecules; Retinoids; rhodopsin; Spectrophotometry, Infrared; Stereoisomerism; rhodopsin; infrared; retinoids; Infrared spectrometry; Fourier transformation; Identification; Retinoids; Technique
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(83)90090-8

The Fourier transform infrared spectra of 15 purified retinoids were compared. Retinoids with conjugated CO groups revealed the presence of a band between 1730 and 1630 cm −1. A characteristic of retinoids is the presence of a band between 1650 and 1620 cm −1 due to CC stretching where three to four conjugated CO and CC bonds were present or between 1610 and 1555 cm −1 where more conjugated unsaturations were present. The presence of cis double bonds was confirmed by a band at 1380 cm −1 while unsubstituted trans double bonds gave absorbances at 990 to 955 cm −1. Epoxy rings, which are present in some retinoids, resulted in bands at 1250, 880, and 790 cm −1 while a furan structure was confirmed by bands at 1175, 1083, and 1065 cm −1.