Experimental design in the development of voltammetric method for the assay of omeprazole

A multivariate strategy was used to optimize an adsorptive stripping voltammetric method for the determination of the antiulcer drug omeprazole. A 3 4 matrix was used for the variable screening while a central composite design was chosen in the subsequent step to evaluate the response surfaces. Simu...

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Published inJournal of pharmaceutical and biomedical analysis Vol. 14; no. 8; pp. 881 - 889
Main Authors Pinzauti, S., Gratteri, P., Furlanetto, S., Mura, P., Dreassi, E., Phan-Tan-Luu, R.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 01.06.1996
Elsevier Science
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Summary:A multivariate strategy was used to optimize an adsorptive stripping voltammetric method for the determination of the antiulcer drug omeprazole. A 3 4 matrix was used for the variable screening while a central composite design was chosen in the subsequent step to evaluate the response surfaces. Simultaneous optimization of the response peak height ( h p) and peak half width w 1 2 ), the latter being a peak shape measure, was achieved. The factors accumulation time, pulse amplitude, scan rate and stirring rate were all found to be statistically significant for the response h p, while for the response w 1 2 only the stirring rate was found to be significant. The optimized method shows a good linearity between peak height and analyte concentration in the concentration range from 8.33 × 10 −9 M to 1.42 × 10 −7 M with a LOD of 6.5 × 10 −9 M. The mean recovery of omeprazole in capsules was 101.9% with a SD of 2.04 (RSD = 200).
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ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(95)01683-X