Experimental design in the development of voltammetric method for the assay of omeprazole

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 14; no. 8; pp. 881 - 889
• Main Authors: Pinzauti, S., Gratteri, P., Furlanetto, S., Mura, P., Dreassi, E., Phan-Tan-Luu, R.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.1996; Elsevier Science
• Subjects: Adsorption; Adsorptive stripping voltammetry; Analysis; Biological and medical sciences; Composite response surface study; Electrochemistry - methods; Experimental design; General pharmacology; Medical sciences; Models, Chemical; Omeprazole - analysis; Omeprazole determination; Pharmacology. Drug treatments; Response surface plot; Response surface plot; Adsorptive stripping voltammetry; Omeprazole determination; Composite response surface study; Experimental design; Omeprazole; Electrochemical method; Antisecretory agent; Dosage form; Voltammetry; Antiulcer agent; Quantitative analysis; Proton pump inhibitor
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/0731-7085(95)01683-X

A multivariate strategy was used to optimize an adsorptive stripping voltammetric method for the determination of the antiulcer drug omeprazole. A 3 4 matrix was used for the variable screening while a central composite design was chosen in the subsequent step to evaluate the response surfaces. Simultaneous optimization of the response peak height ( h p) and peak half width w 1 2 ), the latter being a peak shape measure, was achieved. The factors accumulation time, pulse amplitude, scan rate and stirring rate were all found to be statistically significant for the response h p, while for the response w 1 2 only the stirring rate was found to be significant. The optimized method shows a good linearity between peak height and analyte concentration in the concentration range from 8.33 × 10 −9 M to 1.42 × 10 −7 M with a LOD of 6.5 × 10 −9 M. The mean recovery of omeprazole in capsules was 101.9% with a SD of 2.04 (RSD = 200).