Developmental and immune regulation of gene expression in Hymenolepis diminuta

• Published in: Molecular and biochemical parasitology Vol. 25; no. 1; pp. 19 - 28
• Main Authors: Siddiqui, Afzal A., Karcz, Steve R., Podesta, Ron B.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.08.1987; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Developmental regulation; Diseases caused by cestodes; Electrophoresis, Polyacrylamide Gel; Female; Gene expression; Gene Expression Regulation; Helminthic diseases; Hymenolepis - genetics; Hymenolepis - growth & development; Hymenolepis - immunology; Hymenolepis diminuta; Immune Tolerance; Infectious diseases; Male; Masked mRNA; Medical sciences; Mice; Parasitic diseases; Photofluorography; Protein Biosynthesis; Protein synthesis; Proteins - analysis; Proteins - genetics; Rats; RNA - genetics; RNA, Messenger - genetics; Strobilization; Taeniases; Tropical medicine; Developmental regulation; Protein synthesis; Masked mRNA; HBSS, Hank's balanced salt solution; Strobilization; Hymenolepis diminuta; Gene expression; MEM, minimal essential medium; mRNPs, messenger ribonucleic acid nucleoproteins; Messenger RNA; Animal; Helmintha; Plathelmintha; Immune regulation; Invertebrata; Cestoda
• ISSN: 0166-6851; 1872-9428
• DOI: 10.1016/0166-6851(87)90014-4

Gene expression and its regulation was studied in the rat tapeworm, Hymenolepis diminuta, during strobilization. RNAs extracted from the different developmental stages of the parasite were translated in vitro in a rabbit reticulocyte lysate system. Two-dimensional patterns of the translational products were compared with the 2-dimensional patterns obtained by metabolic labelling with [ 35S]methionine. The results indicated a post-transcriptional regulation of gene expression during strobilization in this parasite. Gene expression and its regulation was also studied in H. diminuta obtained from ‘non-permissive’ hosts (mice) and immune suppressed mice and compared with the parasites of the same age obtained from ‘permissive’ rat hosts. The 2-dimensional patterns of the in vitro translation products, obtained by translating the RNA of different groups of parasites, were compared with the patterns of gene products obtained from parasites using [ 35S]methionine metabolic labelling. The results indicated a massive post-transcriptional regulation of gene expression, the latter inhibited in parasites obtained from normal mice, but not in immune suppressed mice.