Peripheral nerve reconnection: Inhibition of early degenerative processes through the use of a novel fluid medium

• Published in: Experimental neurology Vol. 84; no. 2; pp. 396 - 408
• Main Authors: de Medinaceli, Luis, Church, Allen C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.05.1984; Elsevier
• Subjects: Animals; Axonal Transport - drug effects; Biological and medical sciences; Calcium - physiology; Chlorpromazine - pharmacology; Culture Media - pharmacology; Drug Combinations; Fundamental and applied biological sciences. Psychology; Isolated neuron and nerve. Neuroglia; Nerve Degeneration - drug effects; Polyvinyl Alcohol - pharmacology; Rats; Rats, Inbred Strains; Sciatic Nerve - physiology; Vertebrates: nervous system and sense organs; Wallerian Degeneration - drug effects; PVA; Peripheral nerve; Vertebrata; Mammalia; Wallerian degeneration; Rat; Rodentia
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1016/0014-4886(84)90236-X

Two pharmacologic manipulations were applied to injured nerves in the rat to minimize the secondary damage that accompanies peripheral nerve transection. It is known that calcium influx into the nerve is responsible for some of the processes that have been termed Wallerian degeneration. These disruptive effects of high intracellular calcium were retarded by chlorpromazine, a potent inhibitor of calmodulin. Our results suggested a new method for reducing posttraumatic neural disruption and supported our hypothesis regarding the involvement of calmodulin or some other Ca 2+ binding protein in Wallerian degeneration. The second part of this report describes changes observed at the tips of a severed nerve and their prevention through the use of polyvinyl alcohol. Finally, we showed that neither substance produced functional deficits when injected directly into the sciatic nerve of rats and could thus be used in animal experimentation.