Adjuvant effect of liposomes and adamantylamide dipeptide on antigenicity of entrapped synthetic peptide derived from HIV-1 transmembrane region glycoprotein gp41

• Published in: Immunology letters Vol. 39; no. 2; pp. 157 - 161
• Main Authors: Turánek, Jaroslav, Toman, Miroslav, Novák, Jan, Krchňák, Viktor, Hořavová, Pavla
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.1994; Elsevier
• Subjects: Adamantylamide dipeptide; Adjuvants, Immunologic; AIDS/HIV; Amantadine - analogs & derivatives; Amantadine - immunology; Amino Acid Sequence; Animals; Biological and medical sciences; Dipeptides - immunology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glycoprotein 41; HIV Antibodies - immunology; HIV Antigens - immunology; HIV Envelope Protein gp41 - immunology; HIV-1; HIV-1 - immunology; Humans; Immunobiology; Liposome; Liposomes - immunology; Mice; Mice, Inbred BALB C; Modulation of the immune response (stimulation, suppression); Molecular Sequence Data; Mouse; Peptides - chemical synthesis; Peptides - immunology; Serum Albumin, Bovine; Synthetic peptide antigen; Vaccines, Synthetic - immunology; Adamantylamide dipeptide; HIV-1; Liposome; Glycoprotein 41; Mouse; Synthetic peptide antigen; Performance evaluation; Animal model; Immunization; Antigenic determinant; Rodentia; Retroviridae; Artificial membrane; Virus; Vertebrata; Mammalia; Antigenicity; Immunogenicity; Immunological adjuvant; Lentivirinae; Human immunodeficiency virus; Virus envelope
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/0165-2478(94)90101-5

The synthetic peptide antigen (Ag) (the primary structure Tyr-Leu-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly-Cys-Ser-Gly-Lys-Leu-Ile-Cys-Thr derived from the envelope glycoprotein gp41 of the human immunodeficiency virus type 1 (HIV-1) and exerting specificity with all HIV-1-positive sera available in the Czech Republic (and also in a panel of 10,000 sera from WHO)) was conjugated with bovine serum albumin (BSA) and encapsulated into liposomes. Adjuvant activities of liposomes with various lipid compositions were compared with Freund's complete adjuvant (FCA) and with aluminium hydroxide (AL). The immune response to BSA-Ag liposomes with co-entrapped adamantylamide dipeptide (AdDP) was comparable with that of FCA in terms of longevity and levels of specific antibodies in mouse sera.