Inhibition by cholesterylphosphorylserine of T-cell-mediated immune responses in mice

• Published in: International journal of immunopharmacology Vol. 17; no. 6; pp. 517 - 521
• Main Authors: Bruni, A., Mietto, L., Secchi, F.E., Ponzin, D., Monastra, G., Kirschner, G., Cusinato, F., Chizzolini, C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Science 01.06.1995
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Biological and medical sciences; Cholesterol - analogs & derivatives; Cholesterol - immunology; Cholesterol - pharmacology; cholesterylphosphorylserine; delayed-type hypersensitivity; Graft vs Host Reaction - drug effects; graft-versus-host reaction; Hypersensitivity, Delayed - prevention & control; Immunomodulators; immunoregulation; Lymphocyte Activation - drug effects; Male; Mast Cells - drug effects; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Pharmacology. Drug treatments; Phosphatidylserines - immunology; Phosphatidylserines - pharmacology; Phosphoserine - analogs & derivatives; Phosphoserine - immunology; Phosphoserine - pharmacology; Spleen - cytology; Spleen - drug effects; T-Lymphocytes - drug effects; delayed-type hypersensitivity; immunoregulation; cholesterylphosphorylserine; graft-versus-host reaction; Spleen; Immunopathology; Cell culture; Intraperitoneal administration; Immune response; Rodentia; Graft versus host reaction; Delayed hypersensitivity; In vitro; In vivo; Vertebrata; Chemotherapy; Mammalia; Treatment; Mouse; T-Lymphocyte
• ISSN: 0192-0561; 1879-3495
• DOI: 10.1016/0192-0561(95)00027-Y

The synthetic analogue of phosphatidylserine, cholesterylphosphorylserine (CPHS) inhibits T-cellmediated immune responses in mice. Tested in cultured mouse spleen cells, CPHS inhibits concanavalin A-induced activation of DNA synthesis ( IC 50, 3.5 μM). Injected i.p. during the efferent phase, CPHS (25–100 mg/kg) inhibits the manifestations of delayed-type of hypersensitivity. The compound (25 mg/kg i.p., daily) reduces the acute graft-versus-host reaction when given for 5 days to donor mice before the isolation of spleen cells used for the inoculum. These data suggest that the addition of a phosphorylserine group to a steroid ring may produce immunoregulatory compounds.