Evaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies

• Published in: Journal of veterinary diagnostic investigation Vol. 6; no. 1; p. 30
• Main Authors: Vander Schalie, J, Bradway, D.S, Besser, T.E, Evermann, J.F
• Format: Journal Article
• Language: English
• Published: United States 01.01.1994
• Subjects: Animals; Antibodies, Viral - blood; Arthritis-Encephalitis Virus, Caprine - immunology; Arthritis-Encephalitis Virus, Caprine - isolation & purification; CAPRINE ARTHRITIS ENCEPHAL VIRUS; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Goat Diseases; Goats; Kinetics; Lentivirus Infections - diagnosis; Lentivirus Infections - immunology; Lentivirus Infections - veterinary; Methionine - metabolism; Precipitin Tests - methods; Sensitivity and Specificity; Sulfur Radioisotopes; TEST ELISA; VIRUS ARTHRITE ENCEPHALITE CAPRINE; VIRUS ARTRITIS ENCEFALITIS CAPRINA
• ISSN: 1040-6387; 1943-4936
• DOI: 10.1177/104063879400600106

A kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by immunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in < 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples