Enzyme-linked immunosorbent assay for IgG antibodies to Epstein-Barr virus-associated early antigens and viral capsid antigen

An enzyme-linked immunosorbent assay has been developed for the detection of IgG antibodies to Epstein-Barr virus-associated early antigens and late antigens including the viral capsid antigen. The antibody titers of human sera determined in this way correlate well with those by indirect immunofluor...

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Bibliographic Details
Published inJournal of immunological methods Vol. 67; no. 2; p. 225
Main Authors Dölken, G, Weitzmann, U, Boldt, C, Bitzer, M, Brugger, W, Löhr, G W
Format Journal Article
LanguageEnglish
Published Netherlands 16.03.1984
Subjects
Antigens, Viral - immunology
Capsid - immunology
Cell Line
Cell Nucleus - immunology
Enzyme-Linked Immunosorbent Assay
Epstein-Barr Virus Nuclear Antigens
Fluorescent Antibody Technique
Herpesvirus 4, Human - immunology
Humans
Immunoenzyme Techniques
Immunoglobulin G - analysis
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Summary:An enzyme-linked immunosorbent assay has been developed for the detection of IgG antibodies to Epstein-Barr virus-associated early antigens and late antigens including the viral capsid antigen. The antibody titers of human sera determined in this way correlate well with those by indirect immunofluorescence. ELISA was more sensitive than the IF method. The assays described may be used for rapid and sensitive diagnosis of EBV-related diseases. In addition, the ELISA will be useful for the determination of antibody titers to isolated EBV-associated antigens, e.g., purified components of the EA complex.
ISSN:0022-1759
DOI:10.1016/0022-1759(84)90463-0