Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine

• Published in: Biochimica et biophysica acta Vol. 1037; no. 1; pp. 129 - 132
• Main Authors: Coleman, Richard D., Kim, Tae W., Gotto, Antonio M., Yang, Chao-yuh
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.01.1990; Elsevier; North-Holland
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Apolipoprotein B-100; Apolipoproteins B; Biological and medical sciences; Cysteine; Fluoresceins; Fluorescent labeling; Fundamental and applied biological sciences. Psychology; Humans; Lipoprotein (a); Lipoproteins, LDL; Lipoproteins, myelin; Low density lipoproteins; Male; Molecular Sequence Data; Peptide Fragments - analysis; Proteins; Spectrometry, Fluorescence; Sulfhydryl peptide; Sulfhydryl Reagents; apo; LDL; Low density lipoproteins; Fluorescent labeling; 5-IAF; Amino acid sequence; Sulfhydryl peptide; Apolipoprotein B-100; Lipoprotein (a); HPLC; Lp(a); TFA; Human; Lipoprotein LDL; Cysteine; Fluorescent labelling; Aminoacid; HPLC chromatography; Enzymatic digestion; Apolipoproteins; Localization; Blood; Aminoacid sequence
• ISSN: 0167-4838; 0006-3002; 1879-2588; 1878-2434
• DOI: 10.1016/0167-4838(90)90111-R

Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).