Preliminary crystallographic studies on bovine lactoferrin

• Published in: Journal of molecular biology Vol. 191; no. 1; pp. 143 - 145
• Main Authors: Norris, G.E., Anderson, B.F., Baker, E.N., Baker, H.M., Gärtner, A.L., Ward, J., Rumball, S.V.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 05.09.1986; Elsevier
• Subjects: Animals; Biological and medical sciences; Cattle; Colostrum - metabolism; Crystalline structure; Crystallography; Female; Fundamental and applied biological sciences. Psychology; Humans; Lactoferrin - isolation & purification; Lactoglobulins - isolation & purification; Molecular biophysics; Pregnancy; Structure in molecular biology; Lactoferrin; Unit cell; Purification; Bovine; Crystallization; X ray diffraction; Crystalline structure
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/0022-2836(86)90432-8

The purification of bovine lactoferrin, its crystallization at low ionic strength, and preliminary X-ray crystallographic data are reported. The crystals, which grow from a two-phase system, are radiation-stable and suitable for a medium-resolution X-ray analysis. They are orthorhombic, space group P2 12 12 1, with cell dimensions a = 138.4 A ̊ , b = 87.1 A ̊ , c = 73.6 A ̊ , and one protein molecule in the asymmetric unit.