Canine parainfluenza type 2 and Bordetella bronchiseptica infection produces increased bronchoalveolar lavage thromboxane concentrations in beagle puppies

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 44; no. 3; pp. 171 - 175
• Main Authors: Quan, S.F., Witten, M.L., Dambro, N.N., Lemen, R.J.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.1991; Elsevier
• Subjects: 6-Ketoprostaglandin F1 alpha - blood; 6-Ketoprostaglandin F1 alpha - metabolism; Animals; Biological and medical sciences; Bordetella Infections - complications; Bordetella Infections - metabolism; Bronchoalveolar Lavage Fluid - metabolism; Dogs; Leukotriene B4 - blood; Leukotriene B4 - metabolism; Medical sciences; Parainfluenza Virus 2, Human - metabolism; Paramyxoviridae Infections - complications; Paramyxoviridae Infections - metabolism; Pneumology; Respiratory system : syndromes and miscellaneous diseases; Thromboxane B2 - blood; Thromboxane B2 - metabolism; Fissipedia; Animal model; Carnivora; Respiratory disease; Arachidonic acid derivatives; Exploration; Bronchiolitis; Infection; Bordetella bronchiseptica; Vertebrata; Mammalia; Bronchoalveolar lavage; Animal; Bacteria; Thromboxane; Dog
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/0952-3278(91)90052-7

Acute infections in beagle puppies with canine parainfluenza virus type 2 (CPI2), and CPI2 in combination with Bordetella bronchiseptica (Bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. In order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane A 2 and prostacylin, thromboxane B 2 (TXB 2) and 6-keto-prostaglandin F 1α (6-keto-PGF 1α) respectively, and leukotriene B 4 were measured in the bronchoalveolar lavage (BAL) fluid of 25 beagle puppies (age = 76 ± 1 days, mean ± SEM) 3–4 days after no infection (control, n = 6), inoculation with both CPI2 and Bb (CPI2-Bb, n = 11), inoculation with CPI2 alone (CPI2, n = 4), and inoculation with Bb alone (Bb, n = 4). In addition, plasma levels of TXB 2 and 6-keto-PGF 1α were measured before and after infection in the CPI2-Bb and control groups. The BAL concentration of thromboxane B 2 was increased in the CPI2-Bb group (520 ± 120 pg/ml), but not in the CPI2 (88 ± 40 pg/ml), Bb (235 ± 100 pg/ml), or control groups (120 ± 60 pg/ml, p < 0.01). There also was a borderline increase in BAL concentration of LTB 4 in the CP12-Bb group. No differences were observed in the BAL concentration of 6-keto PGF 1α. Furthermore, neither TXB 2 nor PGF 1α was elevated in the plasma of control or CPI2-Bb puppies. These data suggest that increased thromboxane concentrations in BAL fluid are associated with histamine hyperresponsiveness during acute infection in the CPI2-Bb group.