Effect of bso on the radiation response at low (0–4 Gy) doses

• Published in: International journal of radiation oncology, biology, physics Vol. 22; no. 3; pp. 533 - 536
• Main Authors: Skov, Kirsten A., Susan MacPhail, H.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Elsevier Inc 1992; Elsevier
• Subjects: Animals; Biological and medical sciences; BSO; Buthionine Sulfoximine; Cell Hypoxia - drug effects; Cell Hypoxia - physiology; Cell Hypoxia - radiation effects; CHO Cells; Cricetinae; Glutamate-Cysteine Ligase - antagonists & inhibitors; Glutathione depletion; Low doses; Mammalian cells; Medical sciences; Methionine Sulfoximine - analogs & derivatives; Methionine Sulfoximine - pharmacology; OER; Radiation therapy and radiosensitizing agent; Radiation-Sensitizing Agents - pharmacology; Radiosensitization; Treatment with physical agents; Treatment. General aspects; Tumors; Radiosensitization; Low doses; Glutathione depletion; OER; BSO; Mammalian cells
• ISSN: 0360-3016; 1879-355X
• DOI: 10.1016/0360-3016(92)90869-J

Depletion of thiols by various agents has been shown to radiosensitize hypoxic cells. The agent BSO is used to specifically inhibit GSH synthesis, and the effect of this treatment on radiation response, particularly in the region of clinical interest, was studied using automated microscopic identification of cells for measurement of high survival levels. In both CHO and V79 cell lines, the enhancement rations were greater at low doses (80% S) than at high doses (2% S) in cells irradiated in hypoxia. GSH depletion also affected the aerobic response in both dose regions, with again higher ER's observed at low doses. These results support previous studies which suggest that GSH levels affect the shoulder portion of the survival curve. However, as with untreated cells, the OER's remain higher at high doses than at low doses, in BSO treated cells of both lines. These studies complement an earlier low-dose study on diamide by Skarsgard and co-workers, and were carried out to increase our understanding of the factors which affect radiation sensitivity in the range of doses used in fractionated regimens in the clinic.