Susceptibility of Culicoides variipennis sonorensis to infection by polymerase chain reaction-detectable bluetongue virus in cattle blood

Cattle bloods containing only polymerase chain reaction (PCR)--detectable bluetongue-10 viral nucleic acid, but as determined by virus isolation techniques, not bluetongue-10 virus, were incapable of infecting intrathoracically inoculated Culicoides variipennis sonorensis. These insects also failed...

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Published inThe American journal of tropical medicine and hygiene Vol. 54; no. 5; p. 481
Main Authors Tabachnick, W J, MacLachlan, N J, Thompson, L H, Hunt, G J, Patton, J F
Format Journal Article
LanguageEnglish
Published United States 01.05.1996
Subjects
Animals
Bluetongue - blood
Bluetongue - transmission
Bluetongue - virology
Bluetongue virus
Cattle
Ceratopogonidae - virology
Disease Susceptibility
Eating
Female
Insect Vectors - virology
Polymerase Chain Reaction
Sheep
Survival Rate
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Summary:Cattle bloods containing only polymerase chain reaction (PCR)--detectable bluetongue-10 viral nucleic acid, but as determined by virus isolation techniques, not bluetongue-10 virus, were incapable of infecting intrathoracically inoculated Culicoides variipennis sonorensis. These insects also failed to transmit bluetongue-10 virus when fed on sheep. Cattle whose blood contain only PCR-detectable bluetongue viral nucleic acid, but no infectious virus, are unlikely to play a role in the epidemiology of bluetongue. The biological significance of PCR-based detection assays and their effect on animal health regulations on the international trade of livestock and livestock germplasm is discussed. Bluetongue virus infection provides a very useful model with which to study arthropod-transmitted RNA virus infections of humans and other animals.
ISSN:0002-9637
DOI:10.4269/ajtmh.1996.54.481