Specific transcellular staining of microglia in the adult rat after traumatic degeneration of carbocyanine-filled retinal ganglion cells

• Published in: Experimental eye research Vol. 55; no. 1; pp. 101 - 117
• Main Authors: Thanos, Solon, Pavlidis, Christos, Mey, Jörg, Thiel, Hans-Jürgen
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 01.07.1992; Elsevier
• Subjects: Animals; axotomy; Biological and medical sciences; Carbocyanines; Cell Count; degeneration; Dendrites; Diseases of visual field, optic nerve, optic chiasma and optic tracts; Female; Fluorescent Dyes; ganglion cells; Medical sciences; microglia; Nerve Degeneration; Ophthalmology; Optic Nerve - physiology; Phagocytosis; rat retina; Rats; Rats, Inbred Strains; Retinal Ganglion Cells - cytology; Staining and Labeling - methods; Thiamine Pyrophosphatase - metabolism; Time Factors; phagocytosis; fluorescent dyes; ganglion cells; rat retina; axotomy; microglia; degeneration; Optic nerve; Ganglionic neuron; Rat; Rodentia; Retina; Trauma; Microglia; Visual system; Regeneration; Eye disease; Vertebrata; Axotomy; Mammalia; Animal; Degeneration; Cicatrization; Phagocytosis

The present work was undertaken to assess the fate of ganglion cell debris in the axotomized retina of adult rats and employed a new technique to label phagocytosing microglia via the internalized material. In the main experiment, transection axotomy was performed on the intraorbital segment of the...