Monoclonal Antibodies against Der s 1, a Major Allergen of Dermatophagoides siboney

Six stable clones secreting murine monoclonal antibodies (Mabs) against Der s 1 were obtained. The binding of Mabs showed cross-reactivity with Dermatophagoides farinae, as determined by enzyme-linked immunosorbent assay (ELISA). In a Western blot assay, antibodies reacted with a 24-kD protein consi...

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Published inInternational archives of allergy and immunology Vol. 123; no. 3; pp. 242 - 248
Main Authors Sewer, Minerva, Uyema, Keiko, Labrada, Mayrel, González, Maritza, Coca, Marcos
Format Journal Article
LanguageEnglish
Published Basel, Switzerland S. Karger AG 01.11.2000
Subjects
Allergens - immunology
Allergens - isolation & purification
Allergens - metabolism
Animals
Antibodies, Monoclonal - immunology
Antigens, Dermatophagoides
Blotting, Western
Chromatography, Affinity
Cross Reactions
Der s 1 antigen
Dermatophagoides siboney
Enzyme-Linked Immunosorbent Assay - methods
Epitopes - analysis
Glycoproteins - immunology
Humans
Hypersensitivity - immunology
Immunoglobulin E - immunology
Mites
Original Paper
Sensitivity and Specificity
Mite allergens
Monoclonal antibody
ELISA
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Summary:Six stable clones secreting murine monoclonal antibodies (Mabs) against Der s 1 were obtained. The binding of Mabs showed cross-reactivity with Dermatophagoides farinae, as determined by enzyme-linked immunosorbent assay (ELISA). In a Western blot assay, antibodies reacted with a 24-kD protein considered to represent the major allergen Der s 1. The repertoire of antigenic sites on Der s 1 was studied using a panel of Mabs. Epitope specificity of the Mabs was determined by both competitive inhibition and sandwich ELISA assays. The results defined six different, non-overlapping and non-repeated antigenic sites on the allergen molecule. Der s 1 allergen from Dermatophagoides siboney extracts was purified by Mab affinity chromatography, this procedure gave 43% recovery of >90% pure allergen. The purified allergen had capacity to bind specific human IgE and demonstrated an allergenic activity of up to 77% of total D. siboney extract. An Mab-ELISA was developed using Mabs directed against different epitopes on Der s 1. This assay could detect up to 1 ng/ml of Der s 1 and Der f 1 in allergen preparations.
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ISSN:1018-2438
1423-0097
DOI:10.1159/000024450