Transbilayer migration (flip-flop) of 12-(4-azido-2-nitro-phenoxy)stearoyl glucosamine in large unilamellar phospholipid vesicles
The ability of the glycolipid photoprobe, 12-(4-azido-2-nitrophenoxy)-stearoyl[1- -14C]-glucosamine (12-APS-GlcN), to undergo transbilayer flip-flop and intermembrane transfer between liposomes was examined. It was found that probe which was incorparated into membranes during the preparation of larg...
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Published in | Chemistry and physics of lipids Vol. 40; no. 1; pp. 15 - 21 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.05.1986
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | The ability of the glycolipid photoprobe, 12-(4-azido-2-nitrophenoxy)-stearoyl[1-
-14C]-glucosamine (12-APS-GlcN), to undergo transbilayer flip-flop and intermembrane transfer between liposomes was examined. It was found that probe which was incorparated into membranes during the preparation of large unilamellar vesicles (LUVs) could be rapidly and completely extracted by incubation of these donor vesicles (in the liquid-crystalline state ) with probe-free acceptor vesicles. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-3084 1873-2941 |
DOI: | 10.1016/0009-3084(86)90058-7 |