Transbilayer migration (flip-flop) of 12-(4-azido-2-nitro-phenoxy)stearoyl glucosamine in large unilamellar phospholipid vesicles

The ability of the glycolipid photoprobe, 12-(4-azido-2-nitrophenoxy)-stearoyl[1- -14C]-glucosamine (12-APS-GlcN), to undergo transbilayer flip-flop and intermembrane transfer between liposomes was examined. It was found that probe which was incorparated into membranes during the preparation of larg...

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Published inChemistry and physics of lipids Vol. 40; no. 1; pp. 15 - 21
Main Authors Hu, Valerie W., Greenhut, Susan F., Killeen, Maura P., Roseman, Mark A.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 01.05.1986
Elsevier Science
Subjects
12-(4-azido-2-nitrophenoxy)stearoylglucosamine
Artificial membranes and reconstituted systems
Azides
Biological and medical sciences
flip-flop
Fundamental and applied biological sciences. Psychology
glycolipid photoprobe
intervesicle transfer
Lipid Bilayers
Membrane physicochemistry
Models, Biological
Molecular biophysics
Phosphatidylethanolamines
Pulmonary Surfactants
glycolipid photoprobe
DPPE
12-APS-GlcN
POPC
intervesicle transfer
12-(4-azido-2-nitrophenoxy)stearoylglucosamine
BrPC
LUV
TNBS
flip-flop
DPPC
Vesicle
Phospholipid
Artificial membrane
Glycolipid
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Summary:The ability of the glycolipid photoprobe, 12-(4-azido-2-nitrophenoxy)-stearoyl[1- -14C]-glucosamine (12-APS-GlcN), to undergo transbilayer flip-flop and intermembrane transfer between liposomes was examined. It was found that probe which was incorparated into membranes during the preparation of large unilamellar vesicles (LUVs) could be rapidly and completely extracted by incubation of these donor vesicles (in the liquid-crystalline state ) with probe-free acceptor vesicles.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-3084
1873-2941
DOI:10.1016/0009-3084(86)90058-7