The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands
The application of a new synthetic peptide combinatorial library (SPCL) is described. This library, termed a positional scanning SPCL, contains six positional SPCLs, each of which contains all possible hexameric combinations of 18 of the 20 natural L-amino acids (18 6 = 34, 012, 224 peptides). Each...
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Published in | Life sciences (1973) Vol. 52; no. 18; pp. 1509 - 1517 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Inc
1993
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The application of a new synthetic peptide combinatorial library (SPCL) is described. This library, termed a positional scanning SPCL, contains six positional SPCLs, each of which contains all possible hexameric combinations of 18 of the 20 natural L-amino acids (18
6 = 34, 012, 224 peptides). Each positional SPCL (O
1XXXXX-NH
2, XO
2XXXX-NH
2, XXO
3XXX-NH
2, XXXO
4XX-NH
2, XXXXO
5X-NH
2, and XXXXXO
6-NH
2) was used to determine the most active amino acid for the six positions of a hexamer. Combinations of these amino acids were used to synthesize 24 individual peptides, which were then tested for activity. The most active peptide found corresponded to a hexameric analogue of methionine-enkephalin. Results obtained in this study are compared to those obtained using the SPCL described earlier (1) (O
1O
2XXXX-NH
2), and the subsequent iterative process. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0024-3205 1879-0631 |
DOI: | 10.1016/0024-3205(93)90113-H |