Lipogenesis by Isolated Human Apocrine Sweat Glands: Testosterone Has no Effect During Long-Term Organ Maintenance

• Published in: Journal of investigative dermatology Vol. 92; no. 3; pp. 333 - 336
• Main Authors: Barth, Julian H., Ridden, John, Philpott, Michael P., Greenall, Michael J., Kealey, Terence
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01.03.1989; Nature Publishing
• Subjects: Acetates - metabolism; Adenosine Triphosphate - metabolism; Aged; Apocrine Glands - drug effects; Apocrine Glands - metabolism; Apocrine Glands - ultrastructure; Biological and medical sciences; Chromatography, Thin Layer - methods; Female; Fundamental and applied biological sciences. Psychology; Humans; Lipids - biosynthesis; Lipids - classification; Microscopy, Electron; Middle Aged; Organ Culture Techniques; Sweat Glands - drug effects; Testosterone - pharmacology; Time Factors; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue; Human; Sweat gland; Endocrine gland; Androgen; Lipids; Biosynthesis; Isolated organ; Thin layer chromatography; Testosterone; Apocrine gland; Transmission electron microscopy; Ultrastructure; Skin; Testicular hormone; Sex steroid hormone
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1111/1523-1747.ep12277143

Lipid synthesis by freshly isolated human apocrine glands has been measured by the incorporation of [U-14C] acetate. Incorporation is linear over 6h at 1010 ± 282 pmol/mg wet weight/h (n = 11; mean ± sem). The lipid classes, as percentages of the total lipid synthesized, were found by TLC to be cholesterol 12.3 ± 2.0, mono-glycerides 7.5 ± 1.5, 1,2 di-glycerides 3.0 ± 0.9, 1,3 di-glycerides 3.5 ± 0.5, tri-glycerides 28.4 ± 1.8, free fatty acids 2.0 ± 0.4, lysolecithin 15.4 ± 3.9, sphingomyelin 9.9 ± 4.3, phosphatidyl-choline 8.4 ± 0.4, phosphatidyl-ethanolamine -inositol and -serine 1.8 ± 0.1, phosphatidic acid and cardiolipin 3.3 ± 0.5, and unidentified 3.3 ± 0.5 (mean ± sem, n = 5); Glands were maintained on permeable supports. After 10 d maintenance, electron microscopy showed that the cellular architecture had been preserved, that the ATP contents were the same as in freshly isolated glands, and that [U-14]C] acetate incorporation was not significantly altered at 851 ± 237 pmol/mg/h (n = 18). The addition of 3μM testosterone had no effect on acetate incorporation at 844 ± 231 pmol/mg/h (n = 18). The lipid classes and their proportions were similar to the values for fresh glands after 10 d maintenance both with and without testosterone.