Inhibition of β-N-acetylglucosaminidase by acetamide affects sperm motility and fertilization success of rainbow trout (Oncorhynchus mykiss) and Siberian sturgeon (Acipenser baerii)

• Published in: Theriogenology Vol. 81; no. 5; pp. 723 - 732
• Main Authors: Sarosiek, B., Glogowski, J., Cejko, B.I., Kujawa, R., Szczepkowski, M., Kuźmiński, H., Dobosz, S., Kowalski, R.K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.03.2014
• Subjects: Acetamide; Acetamides - pharmacology; Acetylglucosaminidase - antagonists & inhibitors; Acetylglucosaminidase - physiology; Acrosome - enzymology; Animals; Dose-Response Relationship, Drug; Enzyme Inhibitors - pharmacology; Female; Fertilization; Fertilization - drug effects; Fertilization - physiology; Fishes - physiology; Male; Milt; Oncorhynchus mykiss - physiology; Rainbow trout; Semen - enzymology; Sperm Motility - drug effects; Spermatozoa - enzymology; Spermatozoa - physiology; Sturgeon; β-N-Acetylglucosaminidase; Milt; Sturgeon; β-N-Acetylglucosaminidase; Acetamide; Fertilization; Rainbow trout
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2013.12.006

β-N-Acetylglucosaminidase (β-NAGase) is an enzyme found in the sperm acrosome of numerous animal species including fish. Fish spermatozoa differ in their morphology including acrosome or acrosomeless aquasperm in chondrostean (e.g., sturgeon) and teleostean (e.g., rainbow trout). It has been shown that β-NAGase exists with high activity in both eggs and sperm of these species. The present study shows the potency of β-NAGase in fertilization. In rainbow trout, increase in sperm motility parameters (VAP and MOT) were observed in the presence of acetamide, an inhibitor for β-NAGase. In contrast, sperm motility parameters (VCL, VSL, VAP, MOT, and PRG) were reduced on the Siberian sturgeon in the presence of acetamide. The inhibition of the activity of β-NAGase in rainbow trout spermatozoa was led to a reduction in the number of fertilized eggs from 79% to 40%, whereas in sturgeon no change was observed in fertilization. Moreover, inhibition of β-NAGase in both spermatozoa and eggs of trout and sturgeon resulted in significant decrease in fertilization rate from 79% to 1% in rainbow trout and from 84% to 12% in Siberian sturgeon. Our research proves that β-NAGase can play a significant role in the fertilization process in teleosteans.