Immune response of four dual-CRD C-type lectins to microbial challenges in giant freshwater prawn Macrobrachium rosenbergii

• Published in: Fish & shellfish immunology Vol. 33; no. 2; pp. 155 - 167
• Main Authors: Ren, Qian, Li, Meng, Du, Jie, Zhang, Chi-Yu, Wang, Wen
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2012
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Crustacea - classification; Crustacea - genetics; Freshwater; Gene Expression Profiling; Hepatopancreas - immunology; Immunity, Innate; Innate immunity; Lectin; Lectins, C-Type - chemistry; Lectins, C-Type - genetics; Lectins, C-Type - immunology; Listonella - immunology; Macrobrachium rosenbergii; Microbe; Molecular Sequence Data; Palaemonidae - classification; Palaemonidae - immunology; Positive selection; Sequence Alignment; Up-Regulation; Vibrio - immunology; Vibrio anguillarum; White spot syndrome virus; Lectin; Innate immunity; Microbe; Macrobrachium rosenbergii; Positive selection
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2012.03.009

C-type lectins (CTLs) are believed to play important roles in the innate immunity of invertebrates and serve as pattern recognition receptors, opsonins, or effector molecules. In this study, the full-lengths cDNA of 4 CTL genes from giant freshwater prawn Macrobrachium rosenbergii were cloned and designated as MrLec1, MrLec2, MrLec3, and MrLec4. All of these 4 lectin cDNAs encode proteins with 2 carbohydrate recognition domains (CRDs). While MrLec1, MrLec3, and MrLec4 had signal peptides, no signal peptide was detected in MrLec2. Two carbohydrate recognition motifs within two CRDs of each lectin were predicted (QPE, EPG in MrLec1; EPT, EPA in MrLec2; QPT, NPR in MrLec3; KPN, EPD in MrLec4). Phylogenetic analysis showed that MrLec4 belongs to group A whereas MrLec1, MrLec2, and MrLec3 belong to group B. Positive selection in dual-CRD lectins suggested their probable roles in innate immunity, and positively selected induced amino acid diversity of lectins may confer their ability to recognize a broad range of microbes. The qRT-PCR analysis in adult prawns showed that MrLec1 is mainly expressed in the hepatopancreas, gills, and stomach, MrLec2 and MrLec4 are mainly distributed in the hepatopancreas, and MrLec3 is mainly expressed in the hepatopancreas and stomach. Time-course analysis using qRT-PCR showed that MrLec1 to MrLec4 are all upregulated by the Vibrio anguillarum challenge. MrLec1 is upregulated after 2, 12, and 24 h of white spot syndrome virus (WSSV) challenge. The expression of MrLec2 increases after 12 and 24 h of WSSV challenge, and the transcript of MrLec3 and MrLec4 are downregulated after 2 h of WSSV challenge. The results suggest the potential roles of dual-CRD lectins in the innate immunity of M. rosenbergii. ► Four dual-CRD C-type lectins from Macrobrachium rosenbergii were identified. ► Positive selection of lectins may confer their ability to recognize diverse microbes. ► The expression of four lectins showed diverse responses to microbe challenge.