Separation and functional analysis of eukaryotic DNA topoisomerases by chromatography and electrophoresis

DNA topoisomerases are enzymes that control DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps. Consequently, these enzymes play a crucial role in the regulation of the physiological function of the genome. Beyond their normal functions, topoi...

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Published inJournal of Chromatography B: Biomedical Sciences and Applications Vol. 684; no. 1; pp. 307 - 321
Main Authors Knudsen, Birgitta R., Straub, Tobias, Boege, Fritz
Format Book Review Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 20.09.1996
Subjects
Base Sequence
Chromatography, Liquid - methods
DNA - chemistry
DNA - metabolism
DNA topoisomerases
DNA Topoisomerases, Type I - analysis
DNA Topoisomerases, Type I - isolation & purification
DNA Topoisomerases, Type I - metabolism
Electrophoresis - methods
Enzymes
Eukaryotic Cells - chemistry
Humans
Reviews
Topoisomerases
Enzymes
Reviews
Topoisomerases
DNA topoisomerases
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Summary:DNA topoisomerases are enzymes that control DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps. Consequently, these enzymes play a crucial role in the regulation of the physiological function of the genome. Beyond their normal functions, topoisomerases are important cellular targets in the treatment of human cancers. In this review we summarize current protocols for extracting and purifying DNA topoisomerases, and for separating subtypes and isoforms of these enzymes. Furthermore, we discuss methods for measuring the catalytic activity of topoisomerases and for monitoring the molecular effects of topoisomerase-directed antitumor drugs in cell-free assays.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ObjectType-Review-1
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(96)00152-1