Effects of dihydromyricetin on ARPE‐19 cell migration through regulating matrix metalloproteinase‐2 expression

• Published in: Environmental toxicology Vol. 33; no. 12; pp. 1298 - 1303
• Main Authors: Wang, Kai, Yang, Shun‐Fa, Hsieh, Yi‐Hsien, Chang, Yuan‐Yen, Yu, Nuo‐Yi, Lin, Hui‐Wen, Lin, Hung‐Yu
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.12.2018; Wiley Subscription Services, Inc
• Subjects: adjuvants; Ampelopsis grossedentata; bioactive properties; Cell adhesion & migration; Cell migration; cell movement; Cell Movement - drug effects; Cells; Cells, Cultured; dihydromyricetin (DHM); Epithelial Cells - drug effects; Epithelial Cells - physiology; epithelium; Extracellular; Flavonols - pharmacology; gelatinase A; Humans; JNK protein; Kinases; Matrix metalloproteinase; Matrix Metalloproteinase 2 - metabolism; Metalloproteinase; Metastases; migration; mitogen-activated protein kinase; Mitogen-Activated Protein Kinase 3 - metabolism; Mitogen-Activated Protein Kinases - metabolism; MMP‐2; Phosphorylation; proliferative vitreoretinopathy; Proteins; Retina; Retina - cytology; Retina - drug effects; Retina - metabolism; therapeutics; Vitreoretinopathy, Proliferative - metabolism; Vitreoretinopathy, Proliferative - pathology; Western blotting; migration; MMP-2; dihydromyricetin (DHM); proliferative vitreoretinopathy
• ISSN: 1520-4081; 1522-7278; 1522-7278
• DOI: 10.1002/tox.22637

Dihydromyricetin (DHM), a flavanonol compound in Ampelopsis grossedentata, possesses several biological activities. However, the molecular mechanism underlying the effects of DHM on human proliferative vitreoretinopathy (PVR) remains unclear. We explored the effects of DHM on cell migration and the metastasis‐promoting proteins in human retinal pigment epithelial (RPE) cells (ARPE‐19 cells). Our results revealed that DHM attenuated ARPE‐19 cell invasion and migration by reducing matrix metalloproteinase‐2 (MMP‐2) expression. Furthermore, a Western blot analysis revealed that DHM significantly reduced levels of phosphorylated c‐Jun N‐terminal kinase 1/2, but not those of extracellular signal‐regulated kinase 1/2 and p38. In conclusion, our findings shown that DHM inhibits human RPE cell migration through the inhibition of MMP‐2 expression; therefore, DHM may have potential therapeutic value in treating PVR as adjuvant therapy.