Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells

• Published in: Current Protocols in Neuroscience Vol. 92; no. 1; pp. e96 - n/a
• Main Authors: Zhang, Chi, Kang, Jeong Seuk, Asano, Shoh M., Gao, Ruixuan, Boyden, Edward S.
• Format: Journal Article
• Language: English
• Published: United States 01.06.2020
• Subjects: antibody; beginner; clearing; expansion microscopy; fluorescence microscopy; HeLa cells; hydrogel; imaging; immunocytochemistry; immunohistochemistry; microscopy; microtubules; super‐resolution microscopy; antibody; immunocytochemistry; microtubules; fluorescence microscopy; imaging; clearing; super-resolution microscopy; expansion microscopy; beginner; hydrogel; HeLa cells; microscopy; immunohistochemistry

Expansion microscopy (ExM) is a technique that physically expands preserved cells and tissues before microscope imaging, so that conventional diffraction‐limited microscopes can perform nanoscale‐resolution imaging. In ExM, biomolecules or their markers are linked to a dense, swellable gel network s...