Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells

• Published in: Current Protocols in Neuroscience Vol. 92; no. 1; pp. e96 - n/a
• Main Authors: Zhang, Chi, Kang, Jeong Seuk, Asano, Shoh M., Gao, Ruixuan, Boyden, Edward S.
• Format: Journal Article
• Language: English
• Published: United States 01.06.2020
• Subjects: antibody; beginner; clearing; expansion microscopy; fluorescence microscopy; HeLa cells; hydrogel; imaging; immunocytochemistry; immunohistochemistry; microscopy; microtubules; super‐resolution microscopy; antibody; immunocytochemistry; microtubules; fluorescence microscopy; imaging; clearing; super-resolution microscopy; expansion microscopy; beginner; hydrogel; HeLa cells; microscopy; immunohistochemistry
• ISSN: 1934-8584; 1934-8576
• DOI: 10.1002/cpns.96

Expansion microscopy (ExM) is a technique that physically expands preserved cells and tissues before microscope imaging, so that conventional diffraction‐limited microscopes can perform nanoscale‐resolution imaging. In ExM, biomolecules or their markers are linked to a dense, swellable gel network synthesized throughout a specimen. Mechanical homogenization of the sample (e.g., by protease digestion) and the addition of water enable isotropic swelling of the gel, so that the relative positions of biomolecules are preserved. We previously presented ExM protocols for analyzing proteins and RNAs in cells and tissues. Here we describe a cookbook‐style ExM protocol for expanding cultured HeLa cells with immunostained microtubules, aimed to help newcomers familiarize themselves with the experimental setups and skills required to successfully perform ExM. Our aim is to help beginners, or students in a wet‐lab classroom setting, learn all the key steps of ExM. © 2020 The Authors.